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KD-Validated Anti-Integrin Linked Kinase Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1314
Application
WB, FC, ICC
Uniprot Id
Q13418
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-integrin linked kinase antibody (Cat#ABG1314). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-integrin linked kinase antibody (Cat#ABG1314, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-integrin linked kinase antibody (Cat#ABG1314). Integrin linked kinase expression in wild-type (WT) and integrin linked kinase (DAG1) knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-integrin linked kinase antibody (Cat#ABG1314, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of Integrin linked kinase expression in C2C12 cells using anti-Integrin linked kinase antibody (Cat#ABG1314, 1:2,000). Green, isotype control; red, Integrin linked kinase.
  • Immunocytochemical staining of C2C12 cells with anti-Integrin linked kinase antibody (Cat#ABG1314, 1:1,000). Nuclei were stained blue with DAPI; Integrin linked kinase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1314-100 3 days 100 µL $499.00
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ABG1314-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-Integrin Linked Kinase Rabbit Monoclonal Antibody
Accession (Primary)Q13418
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names25GB2905
Immunogen
A synthesized peptide derived from human ILK
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 51 kDa; observed, 48 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western blotting (WB): 1:1,000-1:5,000Flow Cytometry (FCM): 1:2,000Immunocytochemistry (IC): 1:100-1:1,000

Target Information: ( Q13418 )

Gene NameILK
Other Gene Names
ILK; Integrin Linked Kinase; 59 KDa Serine/Threonine-Protein Kinase; Integrin-Linked Protein Kinase; Beta-Integrin-Linked Kinase; P59ILK; ILK-1; ILK-2; Epididymis Secretory Protein Li 28; Integrin-Linked Kinase-2; Integrin-Linked Kinase; EC 2.7.11.1; HEL-S-28; ILK1; ILK2; P59
About Gene Symbol
ILK (Integrin Linked Kinase) is a serine/threonine protein kinase involved in cell signaling and proliferation. It is an important research target in cancer, cell signaling, and therapeutic target research and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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