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KD-Validated Anti-ATG4B Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1322
Application
WB, FC, ICC
Uniprot Id
Q9Y4P1
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-ATG4B antibody (Cat#ABG1322). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ATG4B antibody (Cat#ABG1322, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-ATG4B antibody (Cat#ABG1322). ATG4B expression in wild type (WT) and ATG4B shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-ATG4B antibody (Cat#ABG1322, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of ATG4B expression in C2C12 cells using ATG4B antibody (Cat#ABG1322, 1:2000). Green, isotype control; red, ATG4B.
  • Immunocytochemical staining of C2C12 cells with ATG4B antibody (Cat#ABG1322, 1:1,000). Nuclei were stained blue with DAPI; ATG4B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1322-100 3 days 100 µL $499.00
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ABG1322-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-ATG4B Rabbit Monoclonal Antibody
Accession (Primary)Q9Y4P1
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names23GB 3240
Immunogen
A synthesized peptide derived from human Atg4B
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 44 kDa , observed, 44 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000

Target Information: ( Q9Y4P1 )

Gene NameATG4B
Other Gene Names
ATG4B; Autophagy Related 4B Cysteine Peptidase; AUTL1; KIAA0943; APG4B; Autophagy-Related Cysteine Endopeptidase 1; Autophagy-Related Protein 4 Homolog B; AUT-Like 1 Cysteine Endopeptidase; Cysteine Protease ATG4B; Autophagin-1; HsAPG4B; HAPG4B; ATG4 Autophagy Related 4 Homolog B (S. Cerevisiae); APG4 Autophagy 4 Homolog B (S. Cerevisiae); Autophagy Related 4B, Cysteine Peptidase; ATG4 Autophagy Related 4 Homolog B; APG4 Autophagy 4 Homolog B; DKFZp586D1822; DKFZP586D1822; EC 3.4.22.-; EC 3.4.22; Apg4B
About Gene Symbol
ATG4B (Autophagy Related 4B Cysteine Peptidase) is a protease enzyme involved in autophagy. It is an important research target in metabolic disease, cancer metabolism, and drug discovery and is widely studied to elucidate cellular mechanisms and identify
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