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KD-Validated Anti-ATP5B Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1323
Application
WB, FC, ICC
Uniprot Id
P06576
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-ATP5B antibody (Cat#ABG1323). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ATP5B antibody (Cat#ABG1323, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-ATP5B antibody (Cat#ABG1323). ATP5B expression in wild type (WT) and ATP5B shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-ATP5B antibody (Cat#ABG1323, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of ATP5B expression in HepG2 cells using ATP5B antibody (Cat#ABG1323, 1:2,000). Green, isotype control; red, ATP5B.
  • Immunocytochemical staining of HepG2 cells with anti-ATP5B antibody (Cat#ABG1323, 1:1000). Nuclei were stained blue with DAPI; ATP5B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1323-100 3 days 100 µL $499.00
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ABG1323-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-ATP5B Rabbit Monoclonal Antibody
Accession (Primary)P06576
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names23GB 3255
Immunogen
A synthesized peptide derived from human ATPB
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 57 kDa , observed, 50 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
WesternBlotting (WB): 1:1,000-1:5,000 Immunocytochemistry (IC): 1:1,000 Flow Cytometry (FCM): 1:2,000

Target Information: ( P06576 )

Gene NameATP5F1B
Other Gene Names
ATP5F1B; ATP Synthase F1 Subunit Beta; ATP5B; ATPSB; ATP Synthase, H+ Transporting, Mitochondrial F1 Complex, Beta Polypeptide; ATP Synthase Subunit Beta, Mitochondrial; ATPMB; Mitochondrial ATP Synthetase, Beta Subunit; Mitochondrial ATP Synthase Beta Subunit; Epididymis Secretory Protein Li 271; EC 3.6.3.14; EC 7.1.2.2; HEL-S-271; EC 3.6.3; HUMOP2
About Gene Symbol
ATP5F1B (ATP Synthase F1 Subunit Beta) is a biosynthetic enzyme involved in cellular metabolism. It is an important research target in metabolic disease, cancer metabolism, and drug discovery and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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