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KD-Validated Anti-MAP2K2 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1324
Application
WB, FC, ICC
Uniprot Id
P36507
Reactivity
Human, Mouse
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-MAP2K2 antibody (Cat#ABG1324). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-MAP2K2 antibody (Cat#ABG1324, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-MAP2K2 antibody (Cat#ABG1324). MAP2K2 expression in wild-type (WT) and MAP2K2 knockdown (KD) 293T cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-MAP2K2 antibody (Cat#ABG1324, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of MAP2K2 expression in HepG2 cells using anti-MAP2K2 antibody (Cat#ABG1324, 1:2,000). Green, isotype control; red, MAP2K2.
  • Immunocytochemical staining of HepG2 cells with anti-MAP2K2 antibody (Cat#ABG1324, 1:1,000). Nuclei were stained blue with DAPI; MAP2K2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1324-100 3 days 100 µL $499.00
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ABG1324-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-MAP2K2 Rabbit Monoclonal Antibody
Accession (Primary)P36507
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names25GB2935
Immunogen
A synthesized peptide derived from human MEK2
Species Reactivity
Reacts WithHuman, Mouse
Calculated MW
Predicted, 44 kDa; observed, 44 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western blotting (WB): 1:1,000-1:5,000Flow Cytometry (FCM): 1:2,000Immunocytochemistry (IC): 1:100-1:1,000

Target Information: ( P36507 )

Gene NameMAP2K2
Other Gene Names
MAP2K2; Mitogen-Activated Protein Kinase Kinase 2; MEK2; MAP Kinase Kinase 2; PRKMK2; MKK2; Dual Specificity Mitogen-Activated Protein Kinase Kinase 2; ERK Activator Kinase 2; MAPK/ERK Kinase 2; EC 2.7.12.2; Mitogen-Activated Protein Kinase Kinase 2, P45; MAPKK 2; MAPKK2; MEK 2; CFC4
About Gene Symbol
MAP2K2 (Mitogen-Activated Protein Kinase Kinase 2) is a mitogen-activated protein kinase involved in MAPK/ERK signaling. It is an important research target in cancer, cell signaling, and therapeutic target research and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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