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KD-Validated Anti-Forkhead Box O3 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1325
Application
WB, FC, ICC
Uniprot Id
O43524
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-forkhead box O3 antibody (Cat#ABG1325). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-forkhead box O3 antibody (Cat#ABG1325, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-forkhead box O3 antibody (Cat#ABG1325). Forkhead box O3 expression in wild-type (WT) and forkhead box O3 (FOXO3) knockdown (KD) HSHC cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-forkhead box O3 antibody (Cat#ABG1325, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of Forkhead box O3 expression in HepG2 cells using anti-Forkhead box O3 antibody (Cat#ABG1325, 1:2,000). Green, isotype control; red, Forkhead box O3.
  • Immunocytochemical staining of HepG2 cells with anti-Forkhead box O3 antibody (Cat#ABG1325, 1:1,000). Nuclei were stained blue with DAPI; Forkhead box O3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1325-100 3 days 100 µL $499.00
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ABG1325-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-Forkhead Box O3 Rabbit Monoclonal Antibody
Accession (Primary)O43524
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names25GB2940
Immunogen
A synthesized peptide derived from human FoxO3a
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 71 kDa; observed, 82 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western blotting (WB): 1:1,000-1:5,000Flow Cytometry (FCM): 1:2,000Immunocytochemistry (IC): 1:100-1:1,000

Target Information: ( O43524 )

Gene NameFOXO3
Other Gene Names
FOXO3; Forkhead Box O3; AF6q21; FKHRL1; FOXO3A; FOXO2; Forkhead In Rhabdomyosarcoma-Like 1; Forkhead Box Protein O3; Forkhead, Drosophila, Homolog Of, In Rhabdomyosarcoma-Like 1; Forkhead Homolog (Rhabdomyosarcoma) Like 1; Forkhead Box O3A; AF6q21 Protein; FKHRL1P2; FOXO3A-
About Gene Symbol
FOXO3 (Forkhead Box O3) is a transcription factor involved in gene transcription and regulation. It is an important research target in cancer research and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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