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KD-Validated Anti-Cell Division Cycle 45 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1327
Application
WB, FC, ICC
Uniprot Id
O75419
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-cell division cycle 45 antibody (Cat#ABG1327). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-cell division cycle 45 antibody (Cat#ABG1327, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-cell division cycle 45 antibody (Cat#ABG1327). Cell division cycle 45 expression in wild type (WT) and cell division cycle 45 (CDC45) knockdown (KD) HSHC cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-cell division cycle 45 antibody (Cat#ABG1327, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of Cell division cycle 45 expression in HepG2 cells using anti-Cell division cycle 45 antibody (Cat#ABG1327, 1:2,000). Green, isotype control; red, Cell division cycle 45.
  • Immunocytochemical staining of HepG2 cells with anti-Cell division cycle 45 antibody (Cat#ABG1327, 1:1,000). Nuclei were stained blue with DAPI; Cell division cycle 45 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1327-100 3 days 100 µL $499.00
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ABG1327-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-Cell Division Cycle 45 Rabbit Monoclonal Antibody
Accession (Primary)O75419
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names25GB2945
Immunogen
A synthesized peptide derived from human CDC45
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 66 kDa; observed, 65 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western blotting (WB): 1:1,000-1:5,000Flow Cytometry (FCM): 1:2,000Immunocytochemistry (IC): 1:100-1:1,000

Target Information: ( O75419 )

Gene NameCDC45
Other Gene Names
CDC45; Cell Division Cycle 45; CDC45L2; CDC45L; Cell Division Control Protein 45 Homolog; Human CDC45; PORC-PI-1; CDC45 (Cell Division Cycle 45, S.Cerevisiae, Homolog)-Like; CDC45 Cell Division Cycle 45-Like (S. Cerevisiae); Cell Division Cycle 45 Homolog (S. Cerevisiae); CDC45 Cell Division Cycle 45 Homolog; CDC45 Cell Division Cycle 45-Like; Cell Division Cycle 45 Homolog; Cell Division Cycle 45-Like 2; CDC45-Related Protein; MGORS7
About Gene Symbol
CDC45 (Cell Division Cycle 45) is a protein kinase and signaling regulator involved in cell signaling and proliferation. It is an important research target in cancer, cell signaling, and therapeutic target research and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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