KD-Validated Anti-DNA Damage Inducible Transcript 3 Rabbit Monoclonal Antibody

Western blotting analysis using anti-DNA damage inducible transcript 3 antibody (Cat#ABG1328). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-DNA damage inducible transcript 3 antibody (Cat#ABG1328, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.

Western blotting analysis using anti-DNA damage inducible transcript 3 antibody (Cat#ABG1328). DNA damage inducible transcript 3 expression in wild-type (WT) and DNA damage inducible transcript 3 (DDIT3) knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-DNA damage inducible transcript 3 antibody (Cat#ABG1328, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.

Flow cytometric analysis of DNA damage inducible transcript 3 expression in HepG2 cells using anti-DNA damage inducible transcript 3c antibody (Cat#ABG1328, 1:2,000). Green, isotype control; red, DNA damage inducible transcript 3.

Immunocytochemical staining of HepG2 cells with anti-DNA damage inducible transcript 3 antibody (Cat#ABG1328, 1:1,000). Nuclei were stained blue with DAPI; DNA damage inducible transcript 3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 µm.