KD-Validated Anti-Nuclear Factor Kappa B Subunit 2 Rabbit Monoclonal Antibody

Western blotting analysis using anti-nuclear factor kappa B subunit 2 antibody (Cat#ABG1336). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-nuclear factor kappa B subunit 2 antibody (Cat#ABG1336, 1:5,000) and HRP-conjugated goat anti rabbit secondary antibody respectively.

Western blotting analysis using anti-nuclear factor kappa B subunit 2 antibody (Cat#ABG1336). Nuclear factor kappa B subunit 2 expression in wild-type (WT) and nuclear factor kappa B subunit 2 (NFKB2) knockdown (KD) HT-1080 cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-nuclear factor kappa B subunit 2 antibody (Cat#ABG1336, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.

Flow cytometric analysis of Nuclear factor kappa B subunit 2 expression in HepG2 cells using anti-Nuclear factor kappa B subunit 2 antibody (Cat#ABG1336, 1:2,000). Green, isotype control; red, Nuclear factor kappa B subunit 2.

lmmunocytochemical staining of HepG2 cells with Nuclear factor kappa B subunit 2 antibody (Cat#ABG1336, 1:1,000). Nuclei were stained blue with DAPl; Nuclear factor kappa B subunit 2 was stained magenta with Alexa Fluor® 647. lmages were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 µm.