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KD-Validated Anti-DDAH1 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1337
Application
WB, FC, ICC
Uniprot Id
O94760
Reactivity
Human
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-dimethylarginine dimethylaminohydrolase 1 antibody (Cat#ABG1337). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-dimethylarginine dimethylaminohydrolase 1 antibody (Cat#ABG1337, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-dimethylarginine dimethylaminohydrolase 1 antibody (Cat#ABG1337). Dimethylarginine dimethylaminohydrolase 1 expression in wild type (WT) and dimethylarginine dimethylaminohydrolase 1 (DDAH1) knockdown (KD) HSHC cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-dimethylarginine dimethylaminohydrolase 1 antibody (Cat#ABG1337, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of Dimethylarginine dimethylaminohydrolase 1 expression in HT-1080 cells using anti-Dimethylarginine dimethylaminohydrolase 1 antibody (Cat#ABG1337, 1:2,000). Green, isotype control; red, Dimethylarginine dimethylaminohydrolase 1.
  • Immunocytochemical staining of HT-1080 cells with anti-Dimethylarginine dimethylaminohydrolase 1 antibody (Cat#ABG1337, 1:1,000). Nuclei were stained blue with DAPI; Dimethylarginine dimethylaminohydrolase 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1337-100 3 days 100 µL $499.00
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ABG1337-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-DDAH1 Rabbit Monoclonal Antibody
Accession (Primary)O94760
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names25GB2995
Immunogen
A synthesized peptide derived from human DDAH1
Species Reactivity
Reacts WithHuman
Calculated MW
Predicted, 31 kDa; observed, 36 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western blotting (WB): 1:1,000-1:5,000Flow Cytometry (FCM): 1:2,000Immunocytochemistry (IC): 1:100-1:1,000

Target Information: ( O94760 )

Gene NameDDAH1
Other Gene Names
DDAH1; Dimethylarginine Dimethylaminohydrolase 1; DDAH; N(G),N(G)-Dimethylarginine Dimethylaminohydrolase 1; Dimethylargininase-1; EC 3.5.3.18; DDAH-1; DDAHI; NG, NG-Dimethylarginine Dimethylaminohydrolase; Epididymis Secretory Protein Li 16; HEL-S-16
About Gene Symbol
DDAH1 (Dimethylarginine Dimethylaminohydrolase 1) is a metabolic enzyme involved in cellular metabolism. It is an important research target in metabolic disease, cancer metabolism, and drug discovery and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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