KD-Validated Anti-MELK Rabbit Monoclonal Antibody

Western blotting analysis using anti-maternal embryonic leucine zipper kinase antibody (Cat#ABG1341). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-maternal embryonic leucine zipper kinase antibody (Cat#ABG1341, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.

Western blotting analysis using anti-maternal embryonic leucine zipper kinase antibody (Cat#ABG1341). Maternal embryonic leucine zipper kinase expression in wild-type (WT) and maternal embryonic leucine zipper kinase (MELK) knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-maternal embryonic leucine zipper kinase antibody (Cat#ABG1341, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.

Flow cytometric analysis of Maternal embryonic leucine zipper kinase expression in HepG2 cells using anti-Maternal embryonic leucine zipper kinase antibody (Cat#ABG1341, 1:2,000). Green, isotype control; red, Maternal embryonic leucine zipper kinase.

Immunocytochemical staining of HepG2 cells with anti-Maternal embryonic leucine zipper kinase antibody (Cat#ABG1341, 1:1,000). Nuclei were stained blue with DAPI; Maternal embryonic leucine zipper kinase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 µm.