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KD-Validated Anti-Myosin IB Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1343
Application
WB, FC, ICC
Uniprot Id
O43795
Reactivity
Human, Mouse
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-myosin IB antibody (Cat#ABG1343). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-myosin IB antibody (Cat#ABG1343, 1:5,000) and HRP-conjugated goat anti rabbit secondary antibody respectively.
  • Western blotting analysis using anti-myosin IB antibody (Cat#ABG1343). Myosin IB expression in wild-type (WT) and myosin IB (MYO1B) knockdown (KD) HT-1080 cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-myosin IB antibody (Cat#ABG1343, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of Myosin IB expression in HepG2 cells using anti-Myosin IBc antibody (Cat#ABG1343, 1:2,000). Green, isotype control; red, Myosin IB.
  • lmmunocytochemical staining of HepG2 cells with Myosin IB antibody (Cat#ABG1343, 1:1,000). Nuclei were stained blue with DAPl; Myosin IB was stained magenta with Alexa Fluor® 647. lmages were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1343-100 3 days 100 µL $499.00
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ABG1343-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-Myosin IB Rabbit Monoclonal Antibody
Accession (Primary)O43795
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names25GB3015
Immunogen
A synthesized peptide derived from human MYO1B
Species Reactivity
Reacts WithHuman, Mouse
Calculated MW
Predicted, 132 kDa; observed, 132 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western blotting (WB): 1:1,000-1:5,000Flow Cytometry (FCM): 1:2,000Immunocytochemistry (IC): 1:100-1:1,000

Target Information: ( O43795 )

Gene NameMYO1B
Other Gene Names
MYO1B; Myosin IB; Myr1; Unconventional Myosin-Ib; MMI-Alpha; MYH-1c; MMI; MYO1B Variant Protein; Myosin-I Alpha; Myosin I Alpha
About Gene Symbol
MYO1B (Myosin IB) is a structural cytoskeletal protein involved in cytoskeletal organization. It is an important research target in cell biology, cancer metastasis, and cytoskeletal research and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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