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KD-Validated Anti-Nth Like DNA Glycosylase 1 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1344
Application
WB, FC, ICC
Uniprot Id
P78549
Reactivity
Human
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-nth like DNA glycosylase 1 antibody (Cat#ABG1344). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-nth like DNA glycosylase 1 antibody (Cat#ABG1344, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-nth like DNA glycosylase 1 antibody (Cat#ABG1344). Nth like DNA glycosylase 1 expression in wild-type (WT) and nth like DNA glycosylase 1 (NTHL1) knockdown (KD) HT-1080 cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-nth like DNA glycosylase 1 antibody (Cat#ABG1344, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of Nth like DNA glycosylase 1 expression in HepG2 cells using anti-Nth like DNA glycosylase 1c antibody (Cat#ABG1344, 1:2,000). Green, isotype control; red, Nth like DNA glycosylase 1.
  • Immunocytochemical staining of Nth like DNA glycosylase 1 cells with Nth like DNA glycosylase 1antibody (Cat#ABG1344, 1:1,000). Nuclei were stained blue with DAPI; Nth like DNA glycosylase 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1344-100 3 days 100 µL $499.00
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ABG1344-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-Nth Like DNA Glycosylase 1 Rabbit Monoclonal Antibody
Accession (Primary)P78549
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names25GB3020
Immunogen
A synthesized peptide derived from human NTH1
Species Reactivity
Reacts WithHuman
Calculated MW
Predicted, 34 kDa; observed, 34 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western blotting (WB): 1:1,000-1:5,000Flow Cytometry (FCM): 1:2,000Immunocytochemistry (IC): 1:100-1:1,000

Target Information: ( P78549 )

Gene NameNTHL1
Other Gene Names
NTHL1; Nth Like DNA Glycosylase 1; OCTS3; NTH1; Bifunctional DNA N-Glycosylase/DNA-(Apurinic Or Apyrimidinic Site) Lyase; Endonuclease III-Like Protein 1; DNA Glycosylase/AP Lyase; HNTH1; Bifunctional DNA N-Glycoslyase/DNA-(Apurinic Or Apyrimidinic Site) Lyase; Nth Endonuclease III-Like 1 (E. Coli); Nth (E.Coli Endonuclease III)-Like 1; Nth Endonuclease III-Like 1; DNA Glycoslyase/AP Lyase; EC 4.2.99.18; EC 3.2.2.-; FAP3
About Gene Symbol
NTHL1 (Nth Like DNA Glycosylase 1) is a metabolic enzyme involved in cellular metabolism. It is an important research target in metabolic disease, cancer metabolism, and drug discovery and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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