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KD-Validated Anti-Mitotic Arrest Deficient 1 Like 1 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1352
Application
WB, FC, ICC
Uniprot Id
Q9Y6D9
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-MAD1L1 antibody (Cat#ABG1352). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-MAD1L1 antibody (Cat#ABG1352, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-MAD1L1 antibody (Cat#ABG1352). MAD1L1 expression in wild-type (WT) and MAD1L1 knockdown (KD) HeLa cells with 20 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-MAD1L1 antibody (Cat#ABG1352, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of MAD1L1 expression in HepG2 cells using anti-MAD1L1 antibody (Cat#ABG1352, 1:2,000). Green, isotype control; red, MAD1L1.
  • Immunocytochemical staining of HepG2 cells with anti-MAD1L1 antibody (Cat#ABG1352, 1:1,000). Nuclei were stained blue with DAPI; MAD1L1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1352-100 3 days 100 µL $499.00
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ABG1352-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-Mitotic Arrest Deficient 1 Like 1 Rabbit Monoclonal Antibody
Accession (Primary)Q9Y6D9
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names25GB3060
Immunogen
A synthesized peptide derived from human MAD1
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 83 kDa; observed, 75 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western blotting (WB): 1:1,000-1:5,000Flow Cytometry (FCM): 1:2,000Immunocytochemistry (IC): 1:100-1:1,000

Target Information: ( Q9Y6D9 )

Gene NameMAD1L1
Other Gene Names
MAD1L1; Mitotic Arrest Deficient 1 Like 1; TXBP181; MAD1; HsMAD1; TP53I9; PIG9; Mitotic Spindle Assembly Checkpoint Protein MAD1; Mitotic Arrest Deficient 1-Like Protein 1; Mitotic Checkpoint MAD1 Protein Homolog; MAD1 Mitotic Arrest Deficient Like 1; Tax-Binding Protein 181; MAD1-Like Protein 1; MAD1 (Mitotic Arrest Deficient, Yeast, Homolog)-Like 1; Mitotic-Arrest Deficient 1, Yeast, Homolog-Like 1; MAD1 Mitotic Arrest Deficient-Like 1 (Yeast); Tumor Protein P53 Inducible Protein 9; HMAD1; MVA7
About Gene Symbol
MAD1L1 (Mitotic Arrest Deficient 1 Like 1) is a transcription factor involved in TGF-β signaling and cell cycle regulation. It is an important research target in cancer research and is widely studied to elucidate cellular mechanisms and identify therapeu
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