KD-Validated Anti-Cytoplasmic linker associated protein 1 Rabbit Monoclonal Antibody

Western blotting analysis using anti-Cytoplasmic linker associated protein 1 antibody (Cat#ABG1356). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Cytoplasmic linker associated protein 1 antibody (Cat#ABG1356, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.

Western blotting analysis using anti-Cytoplasmic linker associated protein 1 antibody (Cat#ABG1356). Cytoplasmic linker associated protein 1 expression in wild type (WT) and Cytoplasmic linker associated protein 1 shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Cytoplasmic linker associated protein 1 antibody (Cat#ABG1356, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.

Flow cytometric analysis of Cytoplasmic linker associated protein 1 expression in HepG2 cells using Cytoplasmic linker associated protein 1 antibody (Cat#ABG1356, 1:2,000). Green, isotype control; red, Cytoplasmic linker associated protein 1.

Immunocytochemical staining of HepG2 cells with Cytoplasmic linker associated protein 1 antibody (Cat#ABG1356, 1:1,000). Nuclei were stained blue with DAPI; Cytoplasmic linker associated protein 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.