KD-Validated Anti-Rho GTPase activating protein 5 Rabbit Monoclonal Antibody

Western blotting analysis using anti-Rho GTPase activating protein 5 antibody (Cat#ABG1361). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Rho GTPase activating protein 5 antibody (Cat#ABG1361, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.

Western blotting analysis using anti-Rho GTPase activating protein 5 antibody (Cat#ABG1361). Rho GTPase activating protein 5 expression in wild type (WT) and Rho GTPase activating protein 5 shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Rho GTPase activating protein 5 antibody (Cat#ABG1361, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.

Flow cytometric analysis of Rho GTPase activating protein 5 expression in C2C12 cells using Rho GTPase activating protein 5 antibody (Cat#ABG1361, 1:2,000). Green, isotype control; red, Rho GTPase activating protein 5.

Immunocytochemical staining of C2C12 cells with Rho GTPase activating protein 5 antibody (Cat#ABG1361, 1:1,000). Nuclei were stained blue with DAPI; Rho GTPase activating protein 5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.