KD-Validated Anti-Oxysterol binding protein like 1A Rabbit Monoclonal Antibody

Western blotting analysis using anti-Oxysterol binding protein like 1A antibody (Cat#ABG1389). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Oxysterol binding protein like 1A antibody (Cat#ABG1389, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.

Western blotting analysis using anti-oxysterol binding protein like 1A antibody (Cat#ABG1389). Oxysterol binding protein like 1A expression in wild-type (WT) and oxysterol binding protein like 1A (OSBPL1A) knockdown (KD) HSHC cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-oxysterol binding protein like 1A antibody (Cat#ABG1389, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.

Flow cytometric analysis of Oxysterol binding protein like 1A expression in HT-1080 cells using Oxysterol binding protein like 1A antibody (Cat#ABG1389, 1:2,000). Green, isotype control; red, Oxysterol binding protein like 1A.

Immunocytochemical staining of HT-1080 cells with Oxysterol binding protein like 1A antibody (Cat#ABG1389, 1:1,000). Nuclei were stained blue with DAPI; Oxysterol binding protein like 1A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.