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KD-Validated Anti-Phospho-STAT1 (S727) Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1409
Application
WB, FC, ICC
Uniprot Id
P42224
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-Phospho-STAT1 (S727) antibody (Cat#ABG1409). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Phospho-STAT1 (S727) antibody (Cat#ABG1409, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-Phospho-STAT1 (S727) antibody (Cat#ABG1409). Phospho-STAT1 (S727) expression in wild type (WT) and Phospho-STAT1 (S727) shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Phospho-STAT1 (S727) antibody (Cat#ABG1409, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of Phospho-STAT1 (S727) expression in HepG2 cells using Phospho-STAT1 (S727) antibody (Cat#ABG1409, 1:2,000). Green, isotype control; red, Phospho-STAT1 (S727).
  • Immunocytochemical staining of HepG2 cells with Phospho-STAT1 (S727) antibody (Cat#ABG1409, 1:1,000). Nuclei were stained blue with DAPI; Phospho-STAT1 (S727) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1409-100 3 days 100 µL $499.00
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ABG1409-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-Phospho-STAT1 (S727) Rabbit Monoclonal Antibody
Accession (Primary)P42224
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names23GB4190
Immunogen
A synthesized peptide derived from human Phospho-STAT1 (S727)
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 87 kDa , observed, 87 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000

Target Information: ( P42224 )

Gene NameSTAT1
Other Gene Names
STAT1; Signal Transducer And Activator Of Transcription 1; Transcription Factor ISGF-3 Components P91/P84; STAT91; ISGF-3; Signal Transducer And Activator Of Transcription 1-Alpha/Beta; Signal Transducer And Activator Of Transcription 1, 91kDa; Signal Transducer And Activator Of Transcription 1, 91kD; CANDF7; IMD31A; IMD31B; IMD31C
About Gene Symbol
STAT1 (Signal Transducer And Activator Of Transcription 1) is a cytokine and immune signaling protein involved in immune signaling. It is an important research target in immunology, inflammation, and cancer research and is widely studied to elucidate cell
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