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KD-Validated Anti-Cyclin Dependent Kinase Inhibitor 2A Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1412
Application
WB, FC, ICC
Uniprot Id
P42771
Reactivity
Human
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-cyclin dependent kinase inhibitor 2A antibody (Cat#ABG1412). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-cyclin dependent kinase inhibitor 2A antibody (Cat#ABG1412, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-cyclin dependent kinase inhibitor 2A antibody (Cat#ABG1412). Cyclin dependent kinase inhibitor 2A expression in wild-type (WT) and cyclin dependent kinase inhibitor 2A (CDKN2A) knockdown (KD) HeLa cells with 30 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-cyclin dependent kinase inhibitor 2A antibody (Cat#ABG1412, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of Cyclin dependent kinase inhibitor 2A expression in HeLa cells using anti-Cyclin dependent kinase inhibitor 2A antibody (Cat#ABG1412, 1:2,000). Green, isotype control; red, Cyclin dependent kinase inhibitor 2A.
  • Immunocytochemical staining of Hela cells with Cyclin dependent kinase inhibitor 2A antibody (Cat#ABG1412, 1:1,000). Nuclei were stained blue with DAPI; Cyclin dependent kinase inhibitor 2A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High.Scale bar, 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1412-100 3 days 100 µL $499.00
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ABG1412-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-Cyclin Dependent Kinase Inhibitor 2A Rabbit Monoclonal Antibody
Accession (Primary)P42771
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names25GB3420
Immunogen
A synthesized peptide derived from human p16 INK
Species Reactivity
Reacts WithHuman
Calculated MW
Predicted, 17 kDa; observed, 17 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western blotting (WB): 1:1,000-1:5,000Flow Cytometry (FCM): 1:2,000Immunocytochemistry (IC): 1:100-1:1,000

Target Information: ( P42771 )

Gene NameCDKN2A
Other Gene Names
CDKN2A; Cyclin Dependent Kinase Inhibitor 2A; P14ARF; CDK4I; MTS1; ARF; Cyclin-Dependent Kinase 4 Inhibitor A; P16-INK4A; P16INK4a; P19Arf; INK4a; CDKN2; CMM2; INK4; P16; P19; P14; MLM; Cyclin-Dependent Kinase Inhibitor 2A (Melanoma, P16,Inhibits CDK4); Cyclin-Dependent Kinase Inhibitor 2A; Multiple Tumour Suppressor 1; Multiple Tumor Suppressor 1; CDKN2A/ARF Intron 2 LncRNA; Alternative Reading Frame; Inhibitor Of Cdk4 A; MTS-1; CAI2; Cell Cycle Negative Regulator Beta; P19 Alternate Open Reading Frame; P14 Alternate Open Reading Frame; CDK4 Inhibitor P16-INK4; Tumor Suppressor ARF; P16-INK4a; P16-INK4; P16INK4A; P16INK4; TP16
About Gene Symbol
CDKN2A (Cyclin Dependent Kinase Inhibitor 2A) is a tumor suppressor protein involved in cell cycle regulation. It is an important research target in cancer research and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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