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KD-Validated Anti-SUPT5H Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1413
Application
WB, FC, ICC
Uniprot Id
O00267
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-SUPT5H antibody (Cat#ABG1413). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-SUPT5H antibody (Cat#ABG1413, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-SUPT5H antibody (Cat#ABG1413). SUPT5H expression in wild type (WT) and SUPT5H shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-SUPT5H antibody (Cat#ABG1413, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of SUPT5H expression in HT-1080 cells using SUPT5H antibody (Cat#ABG1413, 1:2,000). Green, isotype control; red, SUPT5H.
  • Immunocytochemical staining of HT-1080 cells with SUPT5H antibody (Cat#ABG1413, 1:1,000). Nuclei were stained blue with DAPI; SUPT5H was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1413-100 3 days 100 µL $499.00
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ABG1413-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-SUPT5H Rabbit Monoclonal Antibody
Accession (Primary)O00267
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names23GB4220
Immunogen
A synthesized peptide derived from human SUPT5H
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 121 kDa , observed, 155 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000

Target Information: ( O00267 )

Gene NameSUPT5H
Other Gene Names
SUPT5H; SPT5 Homolog, DSIF Elongation Factor Subunit; SPT5H; SPT5; DRB Sensitivity-Inducing Factor 160 KDa Subunit; DRB Sensitivity-Inducing Factor Large Subunit; Transcription Elongation Factor SPT5; Tat-Cotransactivator 1 Protein; DSIF Large Subunit; DSIF P160; FLJ34157; HSPT5; Suppressor Of Ty 5 Homolog (S. Cerevisiae); Suppressor Of Ty (S.Cerevisiae) 5 Homolog; Suppressor Of Ty 5 Homolog; Tat-CT1 Protein; Tat-CT1
About Gene Symbol
SUPT5H (SPT5 Homolog, DSIF Elongation Factor Subunit) is a protein kinase and signaling regulator involved in cell signaling and proliferation. It is an important research target in cancer, cell signaling, and therapeutic target research and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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