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KD-Validated Anti-ATG5 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1424
Application
WB, FC, ICC
Uniprot Id
Q9H1Y0
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-ATG5 antibody (Cat#ABG1424). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ATG5 antibody (Cat#ABG1424, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-ATG5 antibody (Cat#ABG1424). ATG5 expression in wild type (WT) and ATG5 shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-ATG5 antibody (Cat#ABG1424, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of ATG5 expression in HepG2 cells using ATG5 antibody (Cat#ABG1424, 1:2,000). Green, isotype control; red, ATG5.
  • Immunocytochemical staining of HepG2 cells with ATG5 antibody (Cat#ABG1424, 1:1,000). Nuclei were stained blue with DAPI; ATG5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1424-100 3 days 100 µL $499.00
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ABG1424-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-ATG5 Rabbit Monoclonal Antibody
Accession (Primary)Q9H1Y0
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names23GB 1870
Immunogen
A synthesized peptide derived from human ATG5
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 32 kDa , observed, 50 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000

Target Information: ( Q9H1Y0 )

Gene NameATG5
Other Gene Names
ATG5; Autophagy Related 5; ASP; HAPG5; APG5L; APG5; Apoptosis-Specific Protein; Autophagy Protein 5; ATG5 Autophagy Related 5 Homolog (S. Cerevisiae); APG5 (Autophagy 5, S. Cerevisiae)-Like; APG5 Autophagy 5-Like (S. Cerevisiae); ATG5 Autophagy Related 5 Homolog; APG5 Autophagy 5-Like; APG5-LIKE; APG5-Like; SCAR25
About Gene Symbol
ATG5 (Autophagy Related 5) is an autophagy regulatory protein involved in autophagy and apoptosis. It is an important research target in metabolic disease, cancer metabolism, and drug discovery and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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