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KD-Validated Anti-MAP1LC3B Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1428
Application
WB, FC, ICC
Uniprot Id
Q9GZQ8
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-LC3B antibody (Cat#ABG1428). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-LC3B antibody (Cat#ABG1428, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-LC3B antibody (Cat#ABG1428). LC3B expression in wild-type (WT) and MAP1LC3B knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-LC3B antibody (Cat#ABG1428, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of LC3B expression in H9c2 cells using anti-LC3B antibody (Cat#ABG1428, 1:2,000). Green, isotype control; red, LC3B.
  • Immunocytochemical staining of H9C2 cells with anti-LC3B antibody (Cat#ABG1428, 1:1,000). Nuclei were stained blue with DAPI; LC3B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1428-100 3 days 100 µL $499.00
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ABG1428-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-MAP1LC3B Rabbit Monoclonal Antibody
Accession (Primary)Q9GZQ8
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names25GB3515
Immunogen
A synthesized peptide derived from human LC3B
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 15 kDa; observed, 14,16 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western blotting (WB): 1:1,000-1:5,000Flow Cytometry (FCM): 1:2,000Immunocytochemistry (IC): 1:100-1:1,000

Target Information: ( Q9GZQ8 )

Gene NameMAP1LC3B
Other Gene Names
MAP1LC3B; Microtubule Associated Protein 1 Light Chain 3 Beta 2; ATG8F 2; Microtubule-Associated Proteins 1A/1B Light Chain 3B; Autophagy-Related Ubiquitin-Like Modifier LC3 B; MAP1 Light Chain 3-Like Protein 2; MAP1A/MAP1B Light Chain 3 B; MAP1A/MAP1B LC3 B; Microtubule-Associated Protein 1 Light Chain 3 Beta; Autophagy-Related Protein LC3 B; MAP1A/1BLC3; MAP1LC3B-A; MAP1ALC3; LC3B
About Gene Symbol
MAP1LC3B (Microtubule Associated Protein 1 Light Chain 3 Beta 2) is an autophagy regulatory protein involved in autophagy and ubiquitin-proteasome pathway. It is an important research target in metabolic disease, cancer metabolism, and drug discovery and
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