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KD-Validated Anti-Thymidine Kinase 1 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1431
Application
WB, FC, ICC
Uniprot Id
P04183
Reactivity
Human
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-thymidine kinase 1 antibody (Cat#ABG1431). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-thymidine kinase 1 antibody (Cat#ABG1431, 1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-thymidine kinase 1 antibody (Cat#ABG1431). Thymidine kinase 1 expression in wild type (WT) and thymidine kinase 1 (TK1) knockdown (KD) HSHC cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-thymidine kinase 1 antibody (Cat#ABG1431, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of Thymidine kinase 1 expression in HepG2 cells using anti-Thymidine kinase 1 antibody (Cat#ABG1431, 1:2,000). Green, isotype control; red, Thymidine kinase 1.
  • Immunocytochemical staining of HepG2 cells with anti-Thymidine kinase 1 antibody (Cat#ABG1431, 1:1,000). Nuclei were stained blue with DAPI; Thymidine kinase 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1431-100 3 days 100 µL $499.00
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ABG1431-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-Thymidine Kinase 1 Rabbit Monoclonal Antibody
Accession (Primary)P04183
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names25GB3555
Immunogen
A synthesized peptide derived from human Thymidine Kinase 1
Species Reactivity
Reacts WithHuman
Calculated MW
Predicted, 25 kDa; observed, 25 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western blotting (WB): 1:4,000-1:20,000Flow Cytometry (FCM): 1:2,000Immunocytochemistry (IC): 1:100-1:1,000

Target Information: ( P04183 )

Gene NameTK1
Other Gene Names
TK1; Thymidine Kinase 1; Thymidine Kinase, Cytosolic; Cytosolic Thymidine Kinase; EC 2.7.1.21; Epididymis Secretory Sperm Binding Protein; Thymidine Kinase 1, Soluble; TK2
About Gene Symbol
TK1 (Thymidine Kinase 1) is a protein kinase and signaling regulator involved in cell signaling and proliferation. It is an important research target in cancer, cell signaling, and therapeutic target research and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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