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KD-Validated Anti-CREB1 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1433
Application
WB, FC, ICC
Uniprot Id
P16220
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-CREB1 antibody (Cat#ABG1433). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CREB1 antibody (Cat#ABG1433, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-CREB1 antibody (Cat#ABG1433). CREB1 expression in wild type (WT) and CREB1 knockdown (KD) HSHC cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-CREB1 antibody (Cat#ABG1433, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of CREB1 expression in HepG2 cells using anti-CREB1 antibody (Cat#ABG1433, 1:2,000). Green, isotype control; red, CREB1.
  • Immunocytochemical staining of HepG2 cells with anti-CREB1 antibody (Cat#ABG1433, 1:1,000). Nuclei were stained blue with DAPI; CREB1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1433-100 3 days 100 µL $499.00
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ABG1433-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-CREB1 Rabbit Monoclonal Antibody
Accession (Primary)P16220
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names25GB3650
Immunogen
A synthesized peptide derived from human CREB
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 35 kDa; observed, 43-46 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western blotting (WB): 1:1,000-1:5,000Flow Cytometry (FCM): 1:2,000Immunocytochemistry (IC): 1:100-1:1,000

Target Information: ( P16220 )

Gene NameCREB1
Other Gene Names
CAMP Responsive Element Binding Protein 1; Cyclic AMP-Responsive Element-Binding Protein 1; CREB-1; Cyclic Adenosine 3',5'-Monophosphate Response Element-Binding Protein CREB; Cyclic Adenosine 3',5'-Monophosphate Response Element Binding Protein; CAMP-Responsive Element-Binding Protein 1; CAMP-Response Element-Binding Protein-1; Active Transcription Factor CREB; Transactivator Protein; CREB
About Gene Symbol
CREB1 (CAMP Responsive Element Binding Protein 1) is a transcription factor involved in gene transcription and regulation. It is an important research target in gene regulation, development, and cancer research and is widely studied to elucidate cellular
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