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KD-Validated Anti-ERK1/2 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1449
Application
WB, FC, ICC
Uniprot Id
P27361/ P28482
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-ERK1/2 antibody (Cat#ABG1449). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ERK1/2 antibody (Cat#ABG1449, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-ERK1/2 antibody (Cat#ABG1449). ERK1/2 expression in wild type (WT) and ERK1/2 shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-ERK1/2 antibody (Cat#ABG1449, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of ERK1/2 expression in C2C12 cells using ERK1/2 antibody (Cat#ABG1449, 1:2,000). Green, isotype control; red, ERK1/2.
  • Immunocytochemical staining of C2C12 cells with ERK1/2 antibody (Cat#ABG1449, 1:1,000). Nuclei were stained blue with DAPI; ERK1/2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1449-100 3 days 100 µL $499.00
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ABG1449-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-ERK1/2 Rabbit Monoclonal Antibody
Accession (Primary)P27361/ P28482
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names23GB4510
Immunogen
A synthesized peptide derived from human ERK1/2
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 43,41 kDa ; Observed, 42,44 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western blotting (WB): 1:1,000-1:5,000 Flow Cytometry(FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000

Target Information: ( P27361/ P28482 )

Gene NameMAPK1/3
Other Gene Names
MAPK3; Mitogen-Activated Protein Kinase 3; ERK1; PRKM3; Extracellular Signal-Regulated Kinase 1; Microtubule-Associated Protein 2 Kinase; Insulin-Stimulated MAP2 Kinase; EC 2.7.11.24; P44-ERK1; P44-MAPK; P44ERK1; P44MAPK; ERK-1; ERT2; Extracellular Signal-Related Kinase 1; MAP Kinase Isoform P44; MAP Kinase; EC 2.7.11; HS44KDAP; HUMKER1A; P44mapk; P44erk1; MAPK 1; MAPK 3; MAPK1; Mitogen-Activated Protein Kinase 1; ERK2; Extracellular Signal-Regulated Kinase 2; MAPK2; PRKM1; PRKM2; ERK; Mitogen-Activated Protein Kinase 2; MAP Kinase 1; MAP Kinase 2; EC 2.7.11.24; P42-MAPK; P41mapk; MAPK; ERK-2; ERT1; Protein Tyrosine Kinase; ERK2; MAP Kinase Isoform P42; EC 2.7.11; P42MAPK; P41MAPK; MAPK 1; NS13; P38; P40; P41
About Gene Symbol
MAPK1/3 (Mitogen-Activated Protein Kinase 3) is a receptor tyrosine kinase involved in MAPK/ERK signaling. It is an important research target in cancer, cell signaling, and therapeutic target research and is widely studied to elucidate cellular mechanisms
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