KD-Validated Anti-AGPS Rabbit Monoclonal Antibody

Western blotting analysis using anti-alkylglycerone phosphate synthase antibody (Cat#ABG1458). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-alkylglycerone phosphate synthase antibody (Cat#ABG1458, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.

Western blotting analysis using anti-alkylglycerone phosphate synthase antibody (Cat#ABG1458). Alkylglycerone phosphate synthase expression in wild-type (WT) and alkylglycerone phosphate synthase (AGPS) shRNA knockdown (KD) HepG2 cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-alkylglycerone phosphate synthase antibody (Cat#ABG1458, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.

Flow cytometric analysis of Alkylglycerone phosphate synthase expression in HeLa cells using anti-Alkylglycerone phosphate synthase antibody (Cat#ABG1458, 1:2,000). Green, isotype control; red, Alkylglycerone phosphate synthase.

Immunocytochemical staining of Hela cells with anti-Alkylglycerone phosphate synthase antibody (Cat#ABG1458, 1:1,000). Nuclei were stained blue with DAPI; Alkylglycerone phosphate synthase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 µm.