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KD-Validated Anti-Spermine synthase Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1463
Application
WB, FC, ICC
Uniprot Id
P52788
Reactivity
Human
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-Spermine synthase antibody (Cat#ABG1463). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Spermine synthase antibody (Cat#ABG1463, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-Spermine synthase antibody (Cat#ABG1463). Spermine synthase expression in wild type (WT) and Spermine synthase shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Spermine synthase antibody (Cat#ABG1463, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of Spermine synthase expression in HepG2 cells using Spermine synthase antibody (Cat#ABG1463, 1:2,000). Green, isotype control; red, Spermine synthase
  • Immunocytochemical staining of HepG2 cells with Spermine synthase antibody (Cat#ABG1463, 1:1,000). Nuclei were stained blue with DAPI; Spermine synthase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1463-100 3 days 100 µL $499.00
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ABG1463-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-Spermine synthase Rabbit Monoclonal Antibody
Accession (Primary)P52788
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names23GB4640
Immunogen
A synthesized peptide derived from human Spermine synthase
Species Reactivity
Reacts WithHuman
Calculated MW
Predicted, 41 kDa , observed, 41 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000

Target Information: ( P52788 )

Gene NameSMS
Other Gene Names
SMS; Spermine Synthase; SPMSY; MRSR; Spermidine Aminopropyltransferase; SpS; SRS; Snyder-Robinson X-Linked Mental Retardation Syndrome; EC 2.5.1.22; MRXSSR; SPS
About Gene Symbol
SMS (Spermine Synthase) is a biosynthetic enzyme involved in cellular metabolism. It is an important research target in metabolic disease, cancer metabolism, and drug discovery and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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