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KD-Validated Anti-Nesprin3 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1465
Application
WB, ICC
Uniprot Id
Q6ZMZ3
Reactivity
Human
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-Nesprin3 antibody (Cat#ABG1465). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Nesprin3 antibody (Cat#ABG1465, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-Nesprin3 antibody (Cat#ABG1465). Nesprin3 expression in wild type (WT) and Nesprin3 shRNA knockdown (KD) HT-1080 cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Nesprin3 antibody (Cat#ABG1465, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Immunocytochemical staining of HAP-1 cells with Nesprin3 antibody (Cat#ABG1465, 1:1,000). Nuclei were stained blue with DAPI; Nesprin3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1465-100 3 days 100 µL $499.00
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ABG1465-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-Nesprin3 Rabbit Monoclonal Antibody
Accession (Primary)Q6ZMZ3
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names23GB4670
Immunogen
A synthesized peptide derived from human Nesprin3
Species Reactivity
Reacts WithHuman
Calculated MW
Predicted, 112 kDa, observed, 100 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Immunocytochemistry (IC): 1:1,000

Target Information: ( Q6ZMZ3 )

Gene NameSYNE3
Other Gene Names
SYNE3; Spectrin Repeat Containing Nuclear Envelope Family Member 3; Nesprin-3; LINC00341; NET53; Long Intergenic Non-Protein Coding RNA 341; Nuclear Envelope Spectrin Repeat Protein 3; KASH Domain-Containing Protein 3; NCRNA00341; C14orf139; FLJ25605; C14orf49; Nesp3; KASH3; Chromosome 14 Open Reading Frame 139; Chromosome 14 Open Reading Frame 49; Uncharacterized Protein C14orf113; Non-Protein Coding RNA 341; C14ORF139; NESPRIN-3; C14ORF49; NESP3
About Gene Symbol
SYNE3 (Spectrin Repeat Containing Nuclear Envelope Family Member 3) is a structural cytoskeletal protein involved in cytoskeletal organization. It is an important research target in cell biology, cancer metastasis, and cytoskeletal research and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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