KD-Validated Anti-Vasodilator stimulated phosphoprotein Rabbit Monoclonal Antibody

Western blotting analysis using anti-Vasodilator stimulated phosphoprotein antibody (Cat#ABG1470). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Vasodilator stimulated phosphoprotein antibody (Cat#ABG1470, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.

Western blotting analysis using anti-Vasodilator stimulated phosphoprotein antibody (Cat#ABG1470). Vasodilator stimulated phosphoprotein expression in wild type (WT) and Vasodilator stimulated phosphoprotein (VASP) shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Vasodilator stimulated phosphoprotein antibody (Cat#ABG1470, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.

Flow cytometric analysis of Vasodilator stimulated phosphoprotein expression in HT-1080 cells using Vasodilator stimulated phosphoprotein antibody (Cat#ABG1470, 1:2,000). Green, isotype control; red, Vasodilator stimulated phosphoprotein.

Immunocytochemical staining of HT-1080 cells with Vasodilator stimulated phosphoprotein antibody (Cat#ABG1470, 1:1,000). Nuclei were stained blue with DAPI; Vasodilator stimulated phosphoprotein was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.