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KD-Validated Anti-WD repeat domain 1 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1471
Application
WB, FC, ICC
Uniprot Id
O75083
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-WD repeat domain 1 antibody (Cat#ABG1471). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-WD repeat domain 1 antibody (Cat#ABG1471, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-WD repeat domain 1 antibody (Cat#ABG1471). WD repeat domain 1 expression in wild type (WT) and WD repeat domain 1 shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-WD repeat domain 1 antibody (Cat#ABG1471, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of WD repeat domain 1 expression in H9c2 cells using WD repeat domain 1 antibody (Cat#ABG1471, 1:2,000). Green, isotype control; red, WD repeat domain 1.
  • Immunocytochemical staining of H9C2 cells with WD repeat domain 1 antibody (Cat#ABG1471, 1:1,000). Nuclei were stained blue with DAPI; WD repeat domain 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1471-100 3 days 100 µL $499.00
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ABG1471-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-WD repeat domain 1 Rabbit Monoclonal Antibody
Accession (Primary)O75083
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names23GB4720
Immunogen
A synthesized peptide derived from human WDR1
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 66 kDa, observed, 75 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000

Target Information: ( O75083 )

Gene NameWDR1
Other Gene Names
WDR1; WD Repeat Domain 1; AIP1; Actin-Interacting Protein 1; WD Repeat-Containing Protein 1; NORI-1; Epididymis Secretory Protein Li 52; HEL-S-52; PFITS
About Gene Symbol
WDR1 (WD Repeat Domain 1) is a structural cytoskeletal protein involved in cytoskeletal organization. It is an important research target in cell biology, cancer metastasis, and cytoskeletal research and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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