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KD-Validated Anti-Ku70 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1493
Application
WB, FC, ICC
Uniprot Id
P12956
Reactivity
Human
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-Ku70 antibody (Cat#ABG1493). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Ku70 antibody (Cat#ABG1493, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-Ku70 antibody (Cat#ABG1493). Ku70 expression in wild type (WT) and Ku70 shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Ku70 antibody (Cat#ABG1493, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of Ku70 expression in HepG2 cells using Ku70 antibody (Cat#ABG1493, 1:2,000). Green, isotype control; red, Ku70.
  • Immunocytochemical staining of HepG2 cells with anti-Ku70 antibody (Cat #ABG1493, 1:1,000). Nuclei were stained blue with DAPI; Ku70 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1493-100 3 days 100 µL $499.00
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ABG1493-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-Ku70 Rabbit Monoclonal Antibody
Accession (Primary)P12956
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names23GB4855
Immunogen
A synthesized peptide derived from human Ku70
Species Reactivity
Reacts WithHuman
Calculated MW
Predicted, 70 kD a , observed, 70 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000

Target Information: ( P12956 )

Gene NameXRCC6
Other Gene Names
XRCC6; X-Ray Repair Cross Complementing 6; X-Ray Repair Complementing Defective Repair In Chinese Hamster Cells 6; G22P1; KU70; ML8; X-Ray Repair Cross-Complementing Protein 6; Thyroid Autoantigen 70kDa (Ku Antigen); ATP-Dependent DNA Helicase 2 Subunit 1; Thyroid Autoantigen 70kD (Ku Antigen); 5'-Deoxyribose-5-Phosphate Lyase Ku70; Lupus Ku Autoantigen Protein P70; 70 KDa Subunit Of Ku Antigen; DNA Repair Protein XRCC6; Ku Autoantigen, 70kDa; 5'-DRP Lyase Ku70; D22S731; D22S671; CTC75; CTCBF; TLAA; ATP-Dependent DNA Helicase II, 70 KDa Subunit; ATP-Dependent DNA Helicase II 70 KDa Subunit; CTC Box Binding Factor 75 KDa Subunit; CTC Box-Binding Factor 75 KDa Subunit; Thyroid-Lupus Autoantigen P70; Ku Autoantigen P70 Subunit; Thyroid-Lupus Autoantigen; EC 4.2.99.-; EC 3.6.4.-; Ku70
About Gene Symbol
XRCC6 (X-Ray Repair Cross Complementing 6) is an ATP-dependent DNA helicase involved in DNA damage response and repair and adaptive immunity. It is an important research target in autoimmune disease and is widely studied to elucidate cellular mechanisms a
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