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KD-Validated Anti-Aminopeptidase A / CD249 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1518
Application
WB, ICC
Uniprot Id
Q07075
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-Aminopeptidase A / CD249 antibody (Cat#ABG1518). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Aminopeptidase A / CD249 antibody (Cat#ABG1518, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-Aminopeptidase A / CD249 antibody (Cat#ABG1518). Aminopeptidase A / CD249 expression in wild type (WT) and Aminopeptidase A / CD249 shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Aminopeptidase A / CD249 antibody (Cat#ABG1518, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Immunocytochemical staining of HepG2 cells with anti-aminopeptidase A / CD249 antibody (Cat#ABG1518, 1:1,000). Nuclei were stained blue with DAPI; Aminopeptidase A / CD249 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1518-100 3 days 100 µL $499.00
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ABG1518-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-Aminopeptidase A / CD249 Rabbit Monoclonal Antibody
Accession (Primary)Q07075
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names23GB5325
Immunogen
A synthesized peptide derived from human Aminopeptidase A / CD249
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 109 kDa, observed, 250kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB):1:1,000-1:5,000 Immunocytochemistry (IC): 1:1,000

Target Information: ( Q07075 )

Gene NameENPEP
Other Gene Names
ENPEP; Glutamyl Aminopeptidase; CD249; Differentiation Antigen Gp160; Aminopeptidase A; EC 3.4.11.7; Gp160; AP-A; EAP; Glutamyl Aminopeptidase (Aminopeptidase A); CD249 Antigen; EC 3.4.11; GP160; APA
About Gene Symbol
ENPEP (Glutamyl Aminopeptidase) is a protease enzyme involved in adaptive immunity. It is an important research target in immunology, flow cytometry, and cancer immunotherapy and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.

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Research Use Only
For research use only. Not intended for diagnostic or therapeutic procedures.