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KD-Validated Anti-GOPC Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1544
Application
WB, FC, ICC
Uniprot Id
Q9HD26
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-GOPC antibody (Cat#ABG1544). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-GOPC antibody (Cat#ABG1544, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-GOPC antibody (Cat#ABG1544). GOPC expression in wild type (WT) and GOPC shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-GOPC antibody (Cat#ABG1544, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of GOPC expression in HeLa cells using GOPC antibody (Cat#ABG1544, 1:2,000). Green, isotype control; red, GOPC.
  • Immunocytochemical staining of Hela cells with anti-GOPC antibody (Cat#ABG1544, 1:1,000). Nuclei were stained blue with DAPI; GOPC was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1544-100 3 days 100 µL $499.00
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ABG1544-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-GOPC Rabbit Monoclonal Antibody
Accession (Primary)Q9HD26
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names23GB5870
Immunogen
A synthesized peptide derived from human PIST
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 51 kDa , observed , 51 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB):1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000

Target Information: ( Q9HD26 )

Gene NameGOPC
Other Gene Names
GOPC; Golgi Associated PDZ And Coiled-Coil Motif Containing; PIST; FIG; CAL; DJ94G16.2; GOPC1; Golgi-Associated PDZ And Coiled-Coil Motif-Containing Protein; PDZ Protein Interacting Specifically With TC10; CFTR-Associated Ligand; Fused In Glioblastoma; PDZ/Coiled-Coil Domain Binding Partner For The Rho-Family GTPase TC10; Golgi-Associated PDZ And Coiled-Coil Motif Containing Protein; DJ94G16.2 PIST
About Gene Symbol
GOPC (Golgi Associated PDZ And Coiled-Coil Motif Containing) is a protein kinase and signaling regulator involved in Hedgehog signaling. It is an important research target in cancer, cell signaling, and therapeutic target research and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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