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KD-Validated Anti-Lysine demethylase 1B Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1557
Application
WB, FC, ICC
Uniprot Id
Q8NB78
Reactivity
Human, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-Lysine demethylase 1B antibody (Cat#ABG1557). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Lysine demethylase 1B antibody (Cat#ABG1557, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-Lysine demethylase 1B antibody (Cat#ABG1557). Lysine demethylase 1B expression in wild type (WT) and Lysine demethylase 1B shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Lysine demethylase 1B antibody (Cat#ABG1557, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of Lysine demethylase 1B expression in HepG2 cells using Lysine demethylase 1B antibody (Cat#ABG1557, 1:2,000). Green, isotype control; red, Lysine demethylase 1B.
  • Immunocytochemical staining of HepG2 cells with anti-Lysine demethylase 1B antibody (Cat#ABG1557, 1:1,000). Nuclei were stained blue with DAPI; Lysine demethylase 1B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1557-100 3 days 100 µL $499.00
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ABG1557-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-Lysine demethylase 1B Rabbit Monoclonal Antibody
Accession (Primary)Q8NB78
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names23GB6265
Immunogen
A synthesized peptide derived from human LSD2 / AOF1
Species Reactivity
Reacts WithHuman, Rat
Calculated MW
Predicted, 92 kDa , observed, 75 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000

Target Information: ( Q8NB78 )

Gene NameKDM1B
Other Gene Names
KDM1B; Lysine Demethylase 1B; LSD2; AOF1; Flavin-Containing Amine Oxidase Domain-Containing Protein 1; Lysine-Specific Histone Demethylase 1B; Lysine-Specific Histone Demethylase; Lysine (K)-Specific Demethylase 1B; DJ298J15.2; BA204B7.3; FLJ34109; FLJ33898; FLJ43328; C6orf193; Amine Oxidase (Flavin Containing) Domain 1; Chromosome 6 Open Reading Frame 193; Amine Oxidase, Flavin Containing 1; EC 1.14.99.66; C6ORF193
About Gene Symbol
KDM1B (Lysine Demethylase 1B) is a metabolic enzyme involved in chromatin remodeling and epigenetics. It is an important research target in metabolic disease, cancer metabolism, and drug discovery and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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