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KD-Validated Anti-HIF1AN Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1564
Application
WB, FC, ICC
Uniprot Id
Q9NWT6
Reactivity
Human
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-HIF1AN antibody (Cat#ABG1564). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-HIF1AN antibody (Cat#ABG1564, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-HIF1AN antibody (Cat#ABG1564). HIF1AN expression in wild type (WT) and HIF1AN shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-HIF1AN antibody (Cat#ABG1564, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of HIF1AN expression in HeLa cells using HIF1AN antibody (Cat#ABG1564, 1:2,000). GrHIF1ANotype control; red, HIF1AN.
  • Immunocytochemical staining of HeLa cells with anti-HIF1AN antibody (Cat#ABG1564, 1:1,000). Nuclei were stained blue with DAPI; HIF1AN was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1564-100 3 days 100 µL $499.00
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ABG1564-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-HIF1AN Rabbit Monoclonal Antibody
Accession (Primary)Q9NWT6
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names23GB6385
Immunogen
A synthesized peptide derived from human HIF1AN
Species Reactivity
Reacts WithHuman
Calculated MW
Predicted, 40 kDa , observed , 40 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB):1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000

Target Information: ( Q9NWT6 )

Gene NameHIF1AN
Other Gene Names
HIF1AN; Hypoxia Inducible Factor 1 Subunit Alpha Inhibitor; FIH1; Hypoxia-Inducible Factor Asparagine Hydroxylase; Hypoxia-Inducible Factor 1-Alpha Inhibitor; Peptide-Aspartate Beta-Dioxygenase; Factor Inhibiting HIF-1; FLJ20615; FLJ22027; FIH-1; Hypoxia Inducible Factor 1 Alpha Subunit Inhibitor; Factor Inhibiting HIF1; DKFZp762F1811; DKFZP762F1811; EC 1.14.11.30; EC 1.14.11.N4
About Gene Symbol
HIF1AN (Hypoxia Inducible Factor 1 Subunit Alpha Inhibitor) is a metabolic enzyme involved in cellular metabolism. It is an important research target in metabolic disease, cancer metabolism, and drug discovery and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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