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KD-Validated Anti-MLD Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1575
Application
WB, FC, ICC
Uniprot Id
O15121
Reactivity
Human
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-MLD antibody (Cat#ABG1575). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-MLD antibody (Cat#ABG1575, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-MLD antibody (Cat#ABG1575). MLD expression in wild type (WT) and MLD shRNA knockdown (KD) HT-1080 cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-MLD antibody (Cat#ABG1575, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of MLD expression in HepG2 cells using MLD antibody (Cat#ABG1575, 1:2,000). Green, isotype control; red, MLD.
  • Immunocytochemical staining of HT-1080 cells with MLD antibody (Cat#ABG1575, 1:1,000). Nuclei were stained blue with DAPI; MLD was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1575-100 3 days 100 µL $499.00
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ABG1575-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-MLD Rabbit Monoclonal Antibody
Accession (Primary)O15121
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names23GB 1215
Immunogen
A synthesized peptide derived from human MLD
Species Reactivity
Reacts WithHuman
Calculated MW
Predicted, 38 kDa , observed, 34 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000

Target Information: ( O15121 )

Gene NameDEGS1
Other Gene Names
DEGS1; Delta 4-Desaturase, Sphingolipid 1; DES1; MLD; DEGS-1; FADS7; Cell Migration-Inducing Gene 15 Protein; Sphingolipid Delta(4)-Desaturase DES1; Sphingolipid Delta(4)-Desaturase 1; Dihydroceramide Desaturase 1; Membrane Lipid Desaturase; Retinol Isomerase; Des-1; Degenerative Spermatocyte Homolog 1, Lipid Desaturase (Drosophila); Degenerative Spermatocyte Homolog 1, Lipid Desaturase; Degenerative Spermatocyte Homolog, Lipid Desaturase; Membrane Fatty Acid (Lipid) Desaturase; Degenerative Spermatocyte Homolog 1; Migration-Inducing Gene 15 Protein; Sphingolipid Delta 4 Desaturase; Dihydroceramide Desaturase-1; EC 1.14.19.17; EC 5.2.1.-; HLD18; MIG15; DES-1; DEGS
About Gene Symbol
DEGS1 (Delta 4-Desaturase, Sphingolipid 1) is a metabolic enzyme involved in cell migration and invasion. It is an important research target in metabolic disease, cancer metabolism, and drug discovery and is widely studied to elucidate cellular mechanisms
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