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KD-Validated Anti-Neuronal Calcium Sensor 1 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1582
Application
WB, FC, ICC
Uniprot Id
P62166
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-Neuronal calcium sensor 1 antibody (Cat#ABG1582). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Neuronal calcium sensor 1 antibody (Cat#ABG1582, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-Neuronal calcium sensor 1 antibody (Cat#ABG1582). Neuronal calcium sensor 1 expression in wild type (WT) and Neuronal calcium sensor 1 shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Neuronal calcium sensor 1 antibody (Cat#ABG1582, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of Neuronal calcium sensor 1 expression in HepG2 cells using Neuronal calcium sensor 1 antibody (Cat#ABG1582, 1:2,000). Green, isotype control; red, Neuronal calcium sensor 1.
  • Immunocytochemical staining of HepG2 cells with anti-Neuronal calcium sensor 1 antibody (Cat#ABG1582, 1:1,000). Nuclei were stained blue with DAPI; Neuronal calcium sensor 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1582-100 3 days 100 µL $499.00
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ABG1582-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-Neuronal Calcium Sensor 1 Rabbit Monoclonal Antibody
Accession (Primary)P62166
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names24GB25
Immunogen
A synthesized peptide derived from human NCS1
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 22 kDa , observed , 21 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB):1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000

Target Information: ( P62166 )

Gene NameNCS1
Other Gene Names
NCS1; Neuronal Calcium Sensor 1; NCS-1; FREQ; Frequenin-Like Ubiquitous Protein 1; Frequenin-Like Protein 1 ; Frequenin Homolog; FLUP; Frequenin (Drosophila) Homolog; Frequenin Homolog (Drosophila)
About Gene Symbol
NCS1 (Neuronal Calcium Sensor 1) is a protein kinase and signaling regulator involved in cell signaling and proliferation. It is an important research target in cancer, cell signaling, and therapeutic target research and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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