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KD-Validated Anti-MAP Kinase Activating Death Domain Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1587
Application
WB, FC, ICC
Uniprot Id
Q8WXG6
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-MADD antibody (Cat#ABG1587). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-MADD antibody (Cat#ABG1587, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-MADD antibody (Cat#ABG1587). MADD expression in wild type (WT) and MADD shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-MADD antibody (Cat#ABG1587, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of MADD expression in HeLa cells using MADD antibody (Cat#Cat#ABG1587, 1:2,000). Green, isotype control; red, MADD.
  • Immunocytochemical staining of HeLa cells with anti-MADD antibody (Cat#ABG1587, 1:1,000). Nuclei were stained blue with DAPI; MADD was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1587-100 3 days 100 µL $499.00
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ABG1587-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-MAP Kinase Activating Death Domain Rabbit Monoclonal Antibody
Accession (Primary)Q8WXG6
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names24GB110
Immunogen
A synthesized peptide derived from human DENN
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 183 kDa, observed , 230 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB):1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000

Target Information: ( Q8WXG6 )

Gene NameMADD
Other Gene Names
MAP Kinase Activating Death Domain; DENN; Differentially Expressed In Normal And Neoplastic Cells; KIAA0358; RAB3GEP; IG20; MAP Kinase-Activating Death Domain Protein; Insuloma-Glucagonoma Protein 20; Insulinoma Glucagonoma Clone 20; Rab3 GDP/GTP Exchange Protein; Rab3 GDP/GTP Exchange Factor; RabGEF; MAP-Kinase Activating Death Domain; NEDDISH; Rab3GEP; DEEAH
About Gene Symbol
MADD (MAP Kinase Activating Death Domain) is a mitogen-activated protein kinase involved in cell signaling and proliferation. It is an important research target in cancer, cell signaling, and therapeutic target research and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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