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KD-Validated Anti-Mechanistic Target Of Rapamycin Kinase Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1589
Application
WB, FC, ICC
Uniprot Id
P42345
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-mTOR antibody (Cat#ABG1589). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-mTOR antibody (Cat#ABG1589, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-mTOR antibody (Cat#ABG1589). mTOR expression in wild type (WT) and mTOR shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-mTOR antibody (Cat#ABG1589, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of mTOR expression in HepG2 cells using mTOR antibody (Cat#ABG1589, 1:2,000). Green, isotype control; red, mTOR.
  • Immunocytochemical staining of HepG2 cells with anti-mTOR antibody (Cat#ABG1589, 1:1,000). Nuclei were stained blue with DAPI; mTOR was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1589-100 3 days 100 µL $499.00
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ABG1589-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-Mechanistic Target Of Rapamycin Kinase Rabbit Monoclonal Antibody
Accession (Primary)P42345
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names24GB200
Immunogen
A synthesized peptide derived from human mTOR
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 289 kDa , observed , 255 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB):1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000

Target Information: ( P42345 )

Gene NameMTOR
Other Gene Names
Mechanistic Target Of Rapamycin Kinase; RAFT1; RAPT1; Rapamycin And FKBP12 Target 1; Mammalian Target Of Rapamycin; FRAP1; FRAP2; FRAP; FK506-Binding Protein 12-Rapamycin Complex-Associated Protein 1; Mechanistic Target Of Rapamycin (Serine/Threonine Kinase); FK506 Binding Protein 12-Rapamycin Associated Protein 2; FKBP12-Rapamycin Complex-Associated Protein 1; Serine/Threonine-Protein Kinase MTOR; Rapamycin Associated Protein FRAP2; FKBP-Rapamycin Associated Protein; Mechanistic Target Of Rapamycin; Rapamycin Target Protein 1; FLJ44809; DJ576K7.1 (FK506 Binding Protein 12-Rapamycin Associated Protein 1); FK506 Binding Protein 12-Rapamycin Associated Protein 1; FKBP12-Rapamycin Complex-Associated Protein; Rapamycin Target Protein; EC 2.7.11.1; MTOR; SKS
About Gene Symbol
MTOR (Mechanistic Target Of Rapamycin Kinase) is a serine/threonine protein kinase involved in mTOR/PI3K/Akt signaling. It is an important research target in cancer, cell signaling, and therapeutic target research and is widely studied to elucidate cellul
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