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KD-Validated Anti-LYN Proto-Oncogene Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1621
Application
WB, FC, ICC
Uniprot Id
P07948
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-LYN antibody (Cat#ABG1621). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-LYN antibody (Cat#ABG1621, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-LYN antibody (Cat#ABG1621). LYN expression in wild type (WT) and LYN shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-LYN antibody (Cat#ABG1621, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of LYN expression in HepG2 cells using anti-LYN antibody (Cat#ABG1621, 1:2,000). Green, isotype control; red, LYN.
  • Immunocytochemical staining of HepG2 cells with anti-LYN antibody (Cat#ABG1621, 1:1,000). Nuclei were stained blue with DAPI; LYN was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1621-100 3 days 100 µL $499.00
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ABG1621-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-LYN Proto-Oncogene Rabbit Monoclonal Antibody
Accession (Primary)P07948
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names24GB720
Immunogen
A synthesized peptide derived from human Lyn
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 59 kDa , observed , 56 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Immunocytochemistry (IC): 1:100-1:1,000 Flow Cytometry (FCM):1:2,000

Target Information: ( P07948 )

Gene NameLYN
Other Gene Names
LYN Proto-Oncogene, Src Family Tyrosine Kinase; JTK8; V-Yes-1 Yamaguchi Sarcoma Viral Related Oncogene Homolog; Lck/Yes-Related Novel Protein Tyrosine Kinase; Tyrosine-Protein Kinase Lyn; EC 2.7.10.2; P53Lyn; P56Lyn; EC 2.7.10; SAIDV
About Gene Symbol
LYN (LYN Proto-Oncogene, Src Family Tyrosine Kinase) is a receptor tyrosine kinase involved in cell signaling and proliferation. It is an important research target in cancer research and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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