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KD-Validated Anti-Pyruvate Dehydrogenase E2 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1623
Application
WB, FC, ICC
Uniprot Id
P10515
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-Pyruvate Dehydrogenase E2 antibody (Cat#ABG1623). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Pyruvate Dehydrogenase E2 antibody (Cat#ABG1623, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-Pyruvate Dehydrogenase E2 antibody (Cat#ABG1623). Pyruvate Dehydrogenase E2 expression in wild type (WT) and Pyruvate Dehydrogenase E2 shRNA knockdown (KD) HT-1080 cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Pyruvate Dehydrogenase E2 antibody (Cat#ABG1623, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of Pyruvate Dehydrogenase E2 expression in HepG2 cells using Pyruvate Dehydrogenase E2 antibody (Cat#ABG1623, 1:2,000). Green, isotype control; red, Pyruvate Dehydrogenase E2.
  • Immunocytochemical staining of HepG2 cells with Pyruvate Dehydrogenase E2 antibody (Cat#ABG1623, 1:1,000). Nuclei were stained blue with DAPI; Pyruvate Dehydrogenase E2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1623-100 3 days 100 µL $499.00
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ABG1623-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-Pyruvate Dehydrogenase E2 Rabbit Monoclonal Antibody
Accession (Primary)P10515
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names23GB1010
Immunogen
A synthesized peptide derived from human Pyruvate Dehydrogenase E2
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted , 69 kDa , observed , 69 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000

Target Information: ( P10515 )

Gene NameDLAT
Other Gene Names
DLAT; Dihydrolipoamide S-Acetyltransferase; PBC;DLTA; PDC-E2; PDCE2; M2 Antigen Complex 70 KDa Subunit; E2 Component Of Pyruvate Dehydrogenase Complex; EC 2.3.1.12; Dihydrolipoamide Acetyltransferase Component Of Pyruvate Dehydrogenase Complex; Dihydrolipoyllysine-Residue Acetyltransferase Component Of Pyruvate Dehydrogenase Complex Mitochondrial; Pyruvate Dehydrogenase Complex Component E2; 70 KDa Mitochondrial Autoantigen Of Primary Biliary Cirrhosis; EC 2.3.1
About Gene Symbol
DLAT (Dihydrolipoamide S-Acetyltransferase) is a metabolic dehydrogenase involved in adaptive immunity. It is an important research target in fibrotic disease and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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