KD-Validated Anti-PRMT5 Rabbit Monoclonal Antibody

Western blotting analysis using anti-Protein arginine methyltransferase 5 antibody (Cat#ABG1630). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Protein arginine methyltransferase 5 antibody (Cat#ABG1630, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.

Western blotting analysis using anti-protein arginine methyltransferase 5 antibody (Cat#ABG1630). Protein arginine methyltransferase 5 expression in wild-type (WT) and protein arginine methyltransferase 5 (PRMT5) shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-protein arginine methyltransferase 5 antibody (Cat#ABG1630, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.

Flow cytometric analysis of Protein arginine methyltransferase 5 expression in HeLa cells using Protein arginine methyltransferase 5 antibody (Cat#ABG1630, 1:2,000). Green, isotype control; red, Protein arginine methyltransferase 5.

Immunocytochemical staining of Hela cells with anti-Protein arginine methyltransferase 5 antibody (Cat#ABG1630, 1:1,000). Nuclei were stained blue with DAPI; Protein arginine methyltransferase 5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.