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KD-Validated Anti-RAB7 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1633
Application
WB, FC, ICC
Uniprot Id
P51149
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-RAB7A antibody (Cat#ABG1633). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-RAB7A antibody (Cat#ABG1633, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-RAB7A antibody (Cat#ABG1633). RAB7A expression in wild type (WT) and RAB7A shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-RAB7A antibody (Cat#ABG1633, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of RAB7A expression in H9c2 cells using anti-RAB7A antibody (Cat#ABG1633, 1:2,000). Green, isotype control; red, RAB7A.
  • Immunocytochemical staining of H9c2 cells with anti-RAB7A antibody (Cat#ABG1633, 1:1,000). Nuclei were stained blue with DAPI; RAB7A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1633-100 3 days 100 µL $499.00
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ABG1633-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-RAB7 Rabbit Monoclonal Antibody
Accession (Primary)P51149
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names24GB895
Immunogen
A synthesized peptide derived from human RAB7
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 23 kDa , observed , 23 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB):1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000

Target Information: ( P51149 )

Gene NameRAB7A
Other Gene Names
RAB7A, Member RAS Oncogene Family; RAB7; RAB7, Member RAS Oncogene Family; Ras-Related Protein Rab-7a; CMT2B; Charcot-Marie-Tooth Neuropathy 2B; Ras-Associated Protein RAB7; EC 3.6.5.2; PRO2706
About Gene Symbol
RAB7A (RAB7A, Member RAS Oncogene Family) is a small GTPase involved in cell signaling and proliferation. It is an important research target in cancer research and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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