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KD-Validated Anti-SAMM50 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1654
Application
WB, FC, ICC
Uniprot Id
Q9Y512
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-SAMM50 antibody (Cat#ABG1654). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-SAMM50 antibody (Cat#ABG1654, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-SAMM50 antibody (Cat#ABG1654). SAMM50 expression in wild type (WT) and SAMM50 shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-SAMM50 antibody (Cat#ABG1654, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of SAMM50 expression in HeLa cells using SAMM50 antibody (Cat#ABG1654, 1:2,000). Green, isotype control; red, SAMM50.
  • Immunocytochemical staining of HeLa cells with anti-SAMM50 antibody (Cat#ABG1654, 1:1,000). Nuclei were stained blue with DAPI; SAMM50 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:Medium. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1654-100 3 days 100 µL $499.00
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ABG1654-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-SAMM50 Rabbit Monoclonal Antibody
Accession (Primary)Q9Y512
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names24GB1135
Immunogen
A synthesized peptide derived from human SAMM50
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 52 kDa , observed , 52 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB):1:1,000-1:5,000 Immunocytochemistry (IC): 1:100-1:1,000 Flow Cytometry (FCM): 1:2,000

Target Information: ( Q9Y512 )

Gene NameSAMM50
Other Gene Names
SAMM50 Sorting And Assembly Machinery Component; TRG-3; SAM50; YNL026W; CGI-51; OMP85; TOB55; Sorting And Assembly Machinery Component 50 Homolog; Transformation-Related Gene 3 Protein; Sorting And Assembly Machinery Component 50 Homolog (S. Cerevisiae); Sorting And Assembly Machinery 50kDa
About Gene Symbol
SAMM50 (SAMM50 Sorting And Assembly Machinery Component) is a protein kinase and signaling regulator involved in cell signaling and proliferation. It is an important research target in cancer, cell signaling, and therapeutic target research and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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