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KD-Validated Anti-PIK3C3 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1656
Application
WB, FC, ICC
Uniprot Id
Q8NEB9
Reactivity
Human, Mouse
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-PIK3C3 antibody (Cat#ABG1656). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PIK3C3 antibody (Cat#ABG1656, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-PIK3C3 antibody (Cat#ABG1656). PIK3C3 expression in wild type (WT) and PIK3C3 shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-PIK3C3 antibody (Cat#ABG1656, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of PIK3C3 expression in HeLa cells using PIK3C3 antibody (Cat#ABG1656, 1:2,000). Green, isotype control; red, PIK3C3.
  • Immunocytochemical staining of HeLa cells with anti-PIK3C3 antibody (Cat#ABG1656, 1:1,000). Nuclei were stained blue with DAPI; PIK3C3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1656-100 3 days 100 µL $499.00
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ABG1656-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-PIK3C3 Rabbit Monoclonal Antibody
Accession (Primary)Q8NEB9
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names24GB1150
Immunogen
A synthesized peptide derived from human PI 3 Kinase Class 3
Species Reactivity
Reacts WithHuman, Mouse
Calculated MW
Predicted, 102 kDa , observed , 100 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB):1:1,000-1:5,000 Immunocytochemistry (IC): 1:100-1:1,000 Flow Cytometry (FCM): 1:2,000

Target Information: ( Q8NEB9 )

Gene NamePIK3C3
Other Gene Names
Phosphatidylinositol 3-Kinase Catalytic Subunit Type 3; HVps34; Vps34; Phosphatidylinositol 3-Kinase P100 Subunit; Vacuolar Protein Sorting 34 Homolog; Phosphoinositide-3-Kinase, Class 3; PtdIns-3-Kinase Type 3; PI3-Kinase Type 3; EC 2.7.1.137; PI3K Type 3; Phosphatidylinositol 3-Kinase, Catalytic Subunit Type 3; Phosphoinositide-3-Kinase Class 3; EC 2.7.1; VPS34
About Gene Symbol
PIK3C3 (Phosphatidylinositol 3-Kinase Catalytic Subunit Type 3) is a protein kinase and signaling regulator involved in cell signaling and proliferation. It is an important research target in cancer, cell signaling, and therapeutic target research and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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