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KD-Validated Anti-SFPQ Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1662
Application
WB, FC, ICC
Uniprot Id
P23246
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-SFPQ antibody (Cat#ABG1662). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-SFPQ antibody (Cat#ABG1662, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-SFPQ antibody (Cat#ABG1662). SFPQ expression in wild type (WT) and SFPQ shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-SFPQ antibody (Cat#ABG1662, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of SFPQ expression in HepG2 cells using anti-SFPQ antibody (Cat#ABG1662, 1:2,000). Green, isotype control; red, SFPQ.
  • Immunocytochemical staining of HepG2 cells with anti-SFPQ antibody (Cat#ABG1662, 1:1,000). Nuclei were stained blue with DAPI; SFPQ was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1662-100 3 days 100 µL $499.00
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ABG1662-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-SFPQ Rabbit Monoclonal Antibody
Accession (Primary)P23246
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names24GB1220
Immunogen
A synthesized peptide derived from human SFPQ
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 76 kDa , observed , 100 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB):1:1,000-1:5,000 Immunocytochemistry (IC): 1:100-1:1,000 Flow Cytometry (FCM): 1:2,000

Target Information: ( P23246 )

Gene NameSFPQ
Other Gene Names
Splicing Factor Proline And Glutamine Rich; PSF; PPP1R140; Polypyrimidine Tract Binding Protein Associated; Protein Phosphatase 1, Regulatory Subunit 140; DNA-Binding P52/P100 Complex, 100 KDa Subunit; Splicing Factor, Proline- And Glutamine-Rich; Splicing Factor Proline/Glutamine-Rich; 100 KDa DNA-Pairing Protein; Splicing Factor Proline/Glutamine Rich (Polypyrimidine Tract-Binding Protein-Associated); Splicing Factor Proline/Glutamine Rich (Polypyrimidine Tract Binding Protein Associated); Polypyrimidine Tract-Binding Protein-Associated Splicing Factor; Polypyrimidine Tract-Binding Protein-Associated-Splicing Factor; Epididymis Secretory Sperm Binding Protein; PTB-Associated Splicing Factor; PTB-Associated-Splicing Factor; HPOMp100; POMP100
About Gene Symbol
SFPQ (Splicing Factor Proline And Glutamine Rich) is a protein phosphatase involved in cellular metabolism. It is an important research target in metabolic disease, cancer metabolism, and drug discovery and is widely studied to elucidate cellular mechanis
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