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KD-Validated Anti-NHERF1 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1669
Application
WB, FC
Uniprot Id
O14745
Reactivity
Human, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-NHERF Family PDZ Scaffold Protein 1 antibody (Cat#ABG1669). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NHERF Family PDZ Scaffold Protein 1 antibody (Cat#ABG1669, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-EBP50 antibody (Cat#ABG1669). EBP50 expression in wild-type (WT) and NHERF1 shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-EBP50 antibody (Cat#ABG1669, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of EBP50 expression in H9c2 cells using anti-EBP50 antibody (Cat #ABG1669, 1:2,000). Green, isotype control; red, EBP50.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1669-100 3 days 100 µL $499.00
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ABG1669-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-NHERF1 Rabbit Monoclonal Antibody
Accession (Primary)O14745
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names24GB1270
Immunogen
A synthesized peptide derived from human EBP50
Species Reactivity
Reacts WithHuman, Rat
Calculated MW
Predicted, 39 kDa , o bserved , 50 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB):1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000

Target Information: ( O14745 )

Gene NameNHERF1
Other Gene Names
NHERF1; NHERF Family PDZ Scaffold Protein 1; NHERF-1; NHERF; EBP50; SLC9A3R1; NHE-RF; Solute Carrier Family 9, Subfamily A (NHE3, Cation Proton Antiporter 3), Member 3 Regulator 1; Na(+)/H(+) Exchange Regulatory Cofactor NHE-RF1; Ezrin-Radixin-Moesin-Binding Phosphoprotein 50; Regulatory Cofactor Of Na(+)/H(+) Exchanger; Na(+)/H(+) Exchange Regulatory Cofactor 1; SLC9A3 Regulator 1; Solute Carrier Family 9 (Sodium/Hydrogen Exchanger), Isoform 3 Regulatory Factor 1; Solute Carrier Family 9 (Sodium/Hydrogen Exchanger), Isoform 3 Regulator 1; Solute Carrier Family 9 (Sodium/Hydrogen Exchanger), Member 3 Regulator 1; Solute Carrier Family 9 Isoform A3 Regulatory Factor 1; Ezrin-Radixin-Moesin Binding Phosphoprotein-50; Sodium-Hydrogen Exchanger Regulatory Factor 1; Na+/H+ Exchange Regulatory Co-Factor; NPHLOP2
About Gene Symbol
NHERF1 (NHERF Family PDZ Scaffold Protein 1) is a signaling scaffold protein involved in membrane transport. It is an important research target in pharmacology, drug resistance, and membrane biology and is widely studied to elucidate cellular mechanisms a
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