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KD-Validated Anti-RAD21 Cohesin Complex Component Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1678
Application
WB, FC, ICC
Uniprot Id
O60216
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-RAD21 antibody (Cat#ABG1678). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-RAD21 antibody (Cat#ABG1678, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-RAD21 antibody (Cat#ABG1678). RAD21 expression in wild type (WT) and RAD21 shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-RAD21 antibody (Cat#ABG1678, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of RAD21 expression in HepG2 cells using anti-RAD21 antibody (Cat#ABG1678, 1:2,000). Green, isotype control; red, RAD21.
  • Immunocytochemical staining of HepG2 cells with anti-RAD21 antibody (Cat#ABG1678, 1:1,000). Nuclei were stained blue with DAPI; RAD21 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1678-100 3 days 100 µL $499.00
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ABG1678-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-RAD21 Cohesin Complex Component Rabbit Monoclonal Antibody
Accession (Primary)O60216
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names24GB1390
Immunogen
A synthesized peptide derived from human RAD21
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 72 kDa , observed, 130 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB):1:1,000-1:5,000 Immunocytochemistry (IC): 1:100-1:1,000 Flow Cytometry (FCM):1:2,000

Target Information: ( O60216 )

Gene NameRAD21
Other Gene Names
RAD21; RAD21 Cohesin Complex Component; HHR21; SCC1; KIAA0078; Double-Strand-Break Repair Protein Rad21 Homolog; Sister Chromatid Cohesion 1; Nuclear Matrix Protein 1; SCC1 Homolog; Kleisin; NXP-1; HR21; NXP1; Protein Involved In DNA Double-Strand Break Repair; RAD21 (S. Pombe) Homolog; RAD21 Homolog (S. Pombe); RAD21 Homolog; HRAD21; CDLS4; MCD1; MGS
About Gene Symbol
RAD21 (RAD21 Cohesin Complex Component) is a protein kinase and signaling regulator involved in cell signaling and proliferation. It is an important research target in cancer, cell signaling, and therapeutic target research and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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