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KD-Validated Anti-Forkhead box O4 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1686
Application
WB, FC, ICC
Uniprot Id
P98177
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-Forkhead box O4 antibody (Cat#ABG1686). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Forkhead box O4 antibody (Cat#ABG1686, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-Forkhead box O4 antibody (Cat#ABG1686). Forkhead box O4 expression in wild type (WT) and Forkhead box O4 shRNA knockdown (KD) 293T cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Forkhead box O4 antibody (Cat#ABG1686, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of Forkhead box O4 expression in HeLa cells using Forkhead box O4 antibody (Cat#ABG1686, 1:2,000). Green, isotype control; red, Forkhead box O4.
  • Immunocytochemical staining of HeLa cells with Forkhead box O4 antibody (Cat#ABG1686, 1:1,000). Nuclei were stained blue with DAPI; Forkhead box O4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1686-100 3 days 100 µL $499.00
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ABG1686-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-Forkhead box O4 Rabbit Monoclonal Antibody
Accession (Primary)P98177
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names23GB1470
Immunogen
A synthesized peptide derived from human FoxO4
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 54 kDa , observed, 23,65 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000

Target Information: ( P98177 )

Gene NameFOXO4
Other Gene Names
FOXO4; Forkhead Box O4; AFX1; MLLT7; Myeloid/Lymphoid Or Mixed-Lineage Leukemia (Trithorax Homolog, Drosophila); Translocated To, 7 2 3; Fork Head Domain Transcription Factor AFX1; Forkhead Box Protein O4; AFX; Myeloid/Lymphoid Or Mixed-Lineage Leukemia (Trithorax (Drosophila) Homolog); Translocated To, 7
About Gene Symbol
FOXO4 (Forkhead Box O4) is a transcription factor involved in gene transcription and regulation. It is an important research target in cancer research and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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