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KD-Validated Anti-SMARCA4 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1707
Application
WB, FC, ICC
Uniprot Id
P51532
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-SMARCA4 antibody (Cat#ABG1707). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-SMARCA4 antibody (Cat#ABG1707, 1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-SMARCA4 antibody (Cat#ABG1707). SMARCA4 expression in wild type (WT) and SMARCA4 shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-SMARCA4 antibody (Cat#ABG1707, 1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of SMARCA4 expression in HepG2 cells using anti-SMARCA4 antibody (Cat#ABG1707, 1:2,000). Green, isotype control; red, SMARCA4.
  • Immunocytochemical staining of HepG2 cells with anti-SMARCA4 antibody (Cat#ABG1707, 1:1,000). Nuclei were stained blue with DAPI; SMARCA4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1707-100 3 days 100 µL $499.00
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ABG1707-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-SMARCA4 Rabbit Monoclonal Antibody
Accession (Primary)P51532
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names24GB2065
Immunogen
A synthesized peptide derived from human BRG1
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 185 kDa , observed , 185 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:4,000-1:20,000 Immunocytochemistry (IC): 1:100-1:1,000 Flow Cytometry (FCM): 1:2,000

Target Information: ( P51532 )

Gene NameSMARCA4
Other Gene Names
SMARCA4; SWI/SNF Related, Matrix Associated, Actin Dependent Regulator Of Chromatin, Subfamily A, Member 4; BRG1; Mitotic Growth And Transcription Activator; ATP-Dependent Helicase SMARCA4; SNF2-BETA; HSNF2b; BAF190; SNF2LB; SNF2L4; SNF2; SWI2; Global Transcription Activator Homologous Sequence; Sucrose Nonfermenting-Like 4; Transcription Activator BRG1; BRG1-Associated Factor 190A; Protein Brahma Homolog 1; BRM/SWI2-Related Gene 1; Homeotic Gene Regulator; Brahma Protein-Like 1; Nuclear Protein GRB1; Protein BRG-1; SNF2-Like 4; FLJ39786; BAF190A; SWI/SNF-Related Matrix-Associated Actin-Dependent Regulator Of Chromatin Subfamily A Member 4; EC 3.6.4.-; SNF2-Beta; EC 3.6.1; MRD16; RTPS2; SNF2B; CSS4
About Gene Symbol
SMARCA4 (Mitotic Growth And Transcription Activator) is a metabolic enzyme involved in chromatin remodeling and epigenetics. It is an important research target in metabolic disease, cancer metabolism, and drug discovery and is widely studied to elucidate
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