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KD-Validated Anti-Ubiquitination Factor E4B Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1716
Application
WB, FC, ICC
Uniprot Id
O95155
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-Ubiquitination factor E4 B antibody (Cat#ABG1716). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Ubiquitination factor E4 B antibody (Cat#ABG1716, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-Ubiquitination factor E4 B antibody (Cat#ABG1716). Ubiquitination factor E4 B expression in wild type (WT) and Ubiquitination factor E4 B shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Ubiquitination factor E4 B antibody (Cat#ABG1716, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of Ubiquitination factor E4 B expression in HepG2 cells using anti-Ubiquitination factor E4 B antibody (Cat#ABG1716, 1:2,000). Green, isotype control; red, Ubiquitination factor E4 B.
  • Immunocytochemical staining of HepG2 cells with anti-Ubiquitination factor E4 B antibody (Cat#ABG1716, 1:1,000). Nuclei were stained blue with DAPI; Ubiquitination factor E4 B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1716-100 3 days 100 µL $499.00
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ABG1716-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-Ubiquitination Factor E4B Rabbit Monoclonal Antibody
Accession (Primary)O95155
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names24GB1765
Immunogen
A synthesized peptide derived from human UBE4B
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 146 kDa , observed , 130 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB):1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000

Target Information: ( O95155 )

Gene NameUBE4B
Other Gene Names
UBE4B; Ubiquitination Factor E4B; UFD2; KIAA0684; UBOX3; E4; Ubiquitination Factor E4B (UFD2 Homolog, Yeast); RING-Type E3 Ubiquitin Transferase E4 B; Ubiquitin Conjugation Factor E4 B; HDNB1; Ubiquitination Factor E4B (Homologous To Yeast UFD2); Homozygously Deleted In Neuroblastoma-1; Homozygously Deleted In Neuroblastoma 1; Ubiquitin-Fusion Degradation Protein 2; Ubiquitin Fusion Degradation Protein 2; Homologous To Yeast UFD2; EC 2.3.2.27; UFD2A
About Gene Symbol
UBE4B (Ubiquitination Factor E4B) is a metabolic enzyme involved in ubiquitin-proteasome pathway. It is an important research target in metabolic disease, cancer metabolism, and drug discovery and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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